蛋白激酶CK2抑制剂对鸡朊蛋白高表达和抑制表达DF-1细胞生物学行为的影响

doi:10.11843/j.issn.0366-6964.2016.05.016

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (5): 985-992.doi: 10.11843/j.issn.0366-6964.2016.05.016

蛋白激酶CK2抑制剂对鸡朊蛋白高表达和抑制表达DF-1细胞生物学行为的影响

万学瑞，杨润霞，王川，刘桂林，吴润^*

（甘肃农业大学动物医学院，兰州 730070）

收稿日期:2015-09-10 出版日期:2016-05-23 发布日期:2016-05-23
通讯作者: 吴润，男，教授，博士生导师，E-mail：wurun@gsau.edu.cn
作者简介:万学瑞（1979-），女，甘肃白银人，讲师，博士，主要从事预防兽医学研究工作，E-mail：383921499@qq.com
基金资助:
国家自然科学基金（31160510）；甘肃农业大学动物医学院教研产学支持计划（JYCX-KX009）

Effects of CK2 Inhibitor on Biological Behaviour of DF-1 Cells with High Expression and Suppression Expression of Chicken PrP^C

WAN Xue-rui，YANG Run-xia，WANG Chuan，LIU Gui-lin，WU Run^*

（College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070，China）

Received:2015-09-10 Online:2016-05-23 Published:2016-05-23

摘要/Abstract

摘要：

为探讨CK2在鸡细胞型朊蛋白（ChPrP^C）高表达和抑制表达的DF-1细胞增殖、黏附、侵袭和凋亡过程中的作用及其与ChPrP^C表达量的关系，以DF-1-PrP和DF-1-SiRNA-3细胞为模型，DF-1细胞为对照，分别用0、25、50、100 nmol•L^-1米托蒽醌处理以抑制CK2，检测细胞对鼠尾胶原的黏附能力，Transwell小室法检测细胞侵袭力，MTT法检测细胞增殖，流式细胞仪检测细胞凋亡，RT-PCR法检测PRNP基因mRNA转录。结果显示，DF-1-PrP、DF-1-SiRNA-3和DF-1细胞的PRNP基因mRNA转录量随着米托蒽醌浓度的增加均减少，其增殖、黏附、侵袭能力相应下降，而总凋亡率均升高；但在同一米托蒽醌浓度下，DF-1-PrP细胞增殖、黏附、侵袭能力始终高于DF-1细胞，总凋亡率均低于DF-1细胞；DF-1-SiRNA-3细胞则相反。表明ChPrP^C的高表达可促进DF-1细胞增殖、黏附和侵袭，抑制其凋亡，而ChPrP^C的低表达则相反；CK2在ChPrP^C介导 DF-1细胞增殖、黏附、侵袭和凋亡过程中具有重要的作用。本研究结果为进一步阐明ChPrP^C生理功能的分子机制奠定基础。

Abstract:

In order to detect the effect of CK2 on DF-1 cells proliferation，adhesion，invasion and apoptosis based on the higher and lower ChPrP^C expression and its relationship，DF-1 cells with higher expression of chicken PrP^C (DF-1-PrP)，DF-1 cells with suppression expression of chicken PrP^C (DF-1-SiRNA-3) and DF-1 cells were used as cell models，after they were treated with 0，25，50，100 nmol•L^-1 mitoxantrone，adhesion assay，transwell assay，MTT assay，flow cytometric assay and RT-PCR analyses were used to detect cell adhesion，invasion，proliferation，apoptosis and transcription of PRNP mRNA，respectively.The data showed that the expression of PRNP mRNA of DF-1-PrP，DF-1-SiRNA-3 and DF-1 cells were all decreased with the increasing of mitoxantrone concentration，and adhesion，invasion and proliferation ability were reduced，but apoptosis rate were increased.Furthermore，under the same mitoxantrone concentration，adhesion，invasion and proliferation ability of DF-1-PrP cells was higher than DF-1 cells，apoptosis rate was lower，but which of DF-1-SiRNA-3 cells was opposite.Results indicated that higher expression of ChPrP^Cpromoted DF-1 cells adhesion，invasion and proliferation and inhibited apoptosis，but suppression expression of ChPrP^Cwas opposite，CK2 plays an important role in those processes.Data of this study lay the foundation for further clarify the molecular mechanism of ChPrP^C physiological function.

中图分类号:

万学瑞，杨润霞，王川，刘桂林，吴润. 蛋白激酶CK2抑制剂对鸡朊蛋白高表达和抑制表达DF-1细胞生物学行为的影响[J]. 畜牧兽医学报, 2016, 47(5): 985-992.

WAN Xue-rui，YANG Run-xia，WANG Chuan，LIU Gui-lin，WU Run. Effects of CK2 Inhibitor on Biological Behaviour of DF-1 Cells with High Expression and Suppression Expression of Chicken PrP^C[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 985-992.

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蛋白激酶CK2抑制剂对鸡朊蛋白高表达和抑制表达DF-1细胞生物学行为的影响

Effects of CK2 Inhibitor on Biological Behaviour of DF-1 Cells with High Expression and Suppression Expression of Chicken PrP^C

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蛋白激酶CK2抑制剂对鸡朊蛋白高表达和抑制表达DF-1细胞生物学行为的影响

Effects of CK2 Inhibitor on Biological Behaviour of DF-1 Cells with High Expression and Suppression Expression of Chicken PrPC

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Effects of CK2 Inhibitor on Biological Behaviour of DF-1 Cells with High Expression and Suppression Expression of Chicken PrP^C